中国科学院理论物理研究所机构知识库
Advanced  
ITP OpenIR  > 理论物理所1978-2010年知识产出  > 期刊论文
题名: A draft sequence of the rice (Oryza sativa ssp indica) genome
作者: Yu, J ;  Hu, SN ;  Wang, J ;  Li, SG ;  Wong, KSG ;  Liu, B ;  Deng, Y ;  Dai, L ;  Zhou, Y ;  Zhang, XQ ;  Cao, ML ;  Liu, J ;  Sun, JD ;  Tang, JB ;  Chen, YJ ;  Huang, XB ;  Lin, W ;  Ye, C ;  Tong, W ;  Cong, LJ ;  Geng, JN ;  Han, YJ ;  Li, L ;  Li, W ;  Hu, GQ ;  Huang, XG ;  Li, WJ ;  Li, J ;  Liu, ZW ;  Li, L ;  Liu, JP ;  Qi, QH ;  Liu, JS ;  Li, L ;  Wang, XG ;  Lu, H ;  Wu, TT ;  Zhu, M ;  Ni, PX ;  Han, H ;  Dong, W ;  Ren, XY ;  Feng, XL ;  Cui, P ;  Li, XR ;  Wang, H ;  Xu, X ;  Zhai, WX ;  Xu, Z ;  Zhang, JS ;  He, SJ ;  Zhang, JG ;  Xu, JC ;  Zhang, KL ;  Zheng, XW ;  Dong, JH ;  Zeng, WY ;  Tao, L ;  Chen, XW ;  He, J ;  Liu, DF ;  Tian, W ;  Tian, CG ;  Xia, HG ;  Li, G ;  Gao, H ;  Li, P ;  Chen, W ;  Wang, XD ;  Zhang, Y ;  Hu, JF ;  Wang, J ;  Liu, S ;  Yang, J ;  Zhang, GY ;  Xiong, YQ ;  Li, ZJ ;  Mao, L ;  Zhou, CS ;  Zhu, Z ;  Chen, RS ;  Hao, BL ;  Zheng, WM ;  Chen, SY ;  Guo, W ;  Li, GJ ;  Liu, SQ ;  Huang, GY ;  Tao, M ;  Wang, J ;  Zhu, LH ;  Yuan, LP ;  Yang, HM
刊名: CHINESE SCIENCE BULLETIN
出版日期: 2001
卷号: 46, 期号:23, 页码:1937-1942
关键词: DNA ;  CONSTRUCTION ;  LIBRARY
学科分类: Physics
通讯作者: Yang, HM , Chinese Acad Sci, Inst Genet, Beijing 100101, Peoples R China.
部门归属: Chinese Acad Sci, Inst Genet, Beijing 100101, Peoples R China; Chinese Acad Sci, Ctr Genom & Bioinformat, Beijing Genom Inst, Beijing 101300, Peoples R China; Zhejiang Univ, Hangzhou Genom Inst, Inst Bioinformat, Key Lab Bioinformat, Hangzhou 310007, Peoples R China; Natl Hybrid Rice R&D Ctr, Changsha 410125, Peoples R China; Chinese Acad Sci, Inst Biophys, Lab Bioinformat, Beijing 100101, Peoples R China; Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China; Chinese Acad Sci, Inst Theoret Phys, Beijing 100080, Peoples R China; Digital China Ltd, Beijing 100080, Peoples R China; Chinese Acad Sci, Inst Comp Technol, Beijing 100080, Peoples R China; Xi An Jiao Tong Univ, Coll Med, Xian 710061, Peoples R China
英文摘要: The sequence of the rice genome holds fundamental information for its biology, including physiology, genetics, development, and evolution, as well as information on many beneficial phenotypes of economic significance. Using a "whole genome shotgun" approach, we have produced a draft rice genome sequence of Oryza sativa ssp. indica, the major crop rice subspecies in China and many other regions of Asia. The draft genome sequence is constructed from over 4.3 million successful sequencing traces with an accumulative total length of 2214.9 Mb. The initial assembly of the non-redundant sequences reached 409.76 Mb in length, based on 3.30 million successful sequencing traces with a total length of 1797.4 Mb from an indica variant cultivar 93-11, giving an estimated coverage of 95.29% of the rice genome with an average base accuracy of higher than 99%. The coverage of the draft sequence, the randomness of the sequence distribution, and the consistency of BIG-ASSEMBLER, a custom-designed software package used for the initial assembly, were verified rigorously by comparisons against finished BAC clone sequences from both indica and japanica strains, available from the public databases. Over all, 96.3% of full-length cDNAs, 96.4% of STS, STR, RFLP markers, 94.0% of ESTs and 94.9% unigene clusters were identified from the draft sequence. Our preliminary analysis on the data set shows that our rice draft sequence is consistent with the comman standard accepted by the genome sequencing community. The unconditional release of the draft to the public also undoubtedly provides a fundamental resource to the international scientific communities to facilitate genomic and genetic studies on rice biology.
收录类别: SCI
原文出处: 查看原文
WOS记录号: WOS:000173381900001
Citation statistics: 
内容类型: 期刊论文
URI标识: http://ir.itp.ac.cn/handle/311006/13631
Appears in Collections:理论物理所1978-2010年知识产出_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
A draft sequence of the rice.pdf(355KB)----开放获取View Download

Recommended Citation:
Yu, J,Hu, SN,Wang, J,et al. A draft sequence of the rice (Oryza sativa ssp indica) genome[J]. CHINESE SCIENCE BULLETIN,2001,46(23):1937-1942.
Service
 Recommend this item
 Sava as my favorate item
 Show this item's statistics
 Export Endnote File
Google Scholar
 Similar articles in Google Scholar
 [Yu, J]'s Articles
 [Hu, SN]'s Articles
 [Wang, J]'s Articles
CSDL cross search
 Similar articles in CSDL Cross Search
 [Yu, J]‘s Articles
 [Hu, SN]‘s Articles
 [Wang, J]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
  Add to CiteULike  Add to Connotea  Add to Del.icio.us  Add to Digg  Add to Reddit 
文件名: A draft sequence of the rice.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  中国科学院理论物理研究所 - Feedback
Powered by CSpace