ITP OpenIR  > 理论物理所科研产出  > SCI论文
Ou-Yang, ZC; Zhou, HJ; Zhang, Y , Donald Danforth Plant Sci Ctr, Lab Comp Genom, 893 N Warson Rd, St Louis, MO 63141 USA.
Stretching single-stranded DNA: Interplay of electrostatic, base-pairing, and base-pair stacking interactions
Source PublicationBIOPHYSICAL JOURNAL
Keyword16s Supercoiled Dna Molecules Elasticity Polymerase Mechanics Dynamics Systems Force 16s
AbstractRecent single-macromolecule observations revealed that the force/extension characteristics of single-stranded DNA (ssDNA) are closely related to solution ionic concentration and DNA sequence composition. To understand this, we studied the elastic property of ssDNA through the Monte Carlo implementation of a modified freely jointed chain (FJC), with electrostatic, base-pairing, and base-pair stacking interactions all incorporated. The simulated force-extension profiles for both random and designed sequences have attained quantitative agreements with the experimental data. In low-salt solution, electrostatic interaction dominates, and at low forces, the molecule can be more easily aligned than an unmodified FJC. In high-salt solution, secondary hairpin structure appears in ssDNA by the formation of base pairs between complementary bases, and external stretching causes a hairpin-coil structural transition, which is continuous for ssDNA made of random sequences. In designed sequences such as poly(dA-dT) and poly(dG-dC), the stacking potential between base pairs encourages the aggregation of base pairs into bulk hairpins and makes the hairpin-coil transition a discontinuous (first-order) process. The sensitivity of elongation to the base-pairing rule is also investigated. The comparison of modeling calculations and the experimental data suggests that the base pairing of single-stranded polynucleotide molecules tends to form a nested and independent planar hairpin structure rather than a random intersecting pattern.
2001
ISSN0006-3495
Volume81Issue:2Pages:1133-1143
Subject AreaPhysics
Indexed BySCI
Citation statistics
Document Type期刊论文
Identifierhttp://ir.itp.ac.cn/handle/311006/13690
Collection理论物理所科研产出_SCI论文
Corresponding AuthorZhang, Y , Donald Danforth Plant Sci Ctr, Lab Comp Genom, 893 N Warson Rd, St Louis, MO 63141 USA.
Recommended Citation
GB/T 7714
Ou-Yang, ZC,Zhou, HJ,Zhang, Y , Donald Danforth Plant Sci Ctr, Lab Comp Genom, 893 N Warson Rd, St Louis, MO 63141 USA.. Stretching single-stranded DNA: Interplay of electrostatic, base-pairing, and base-pair stacking interactions[J]. BIOPHYSICAL JOURNAL,2001,81(2):1133-1143.
APA Ou-Yang, ZC,Zhou, HJ,&Zhang, Y , Donald Danforth Plant Sci Ctr, Lab Comp Genom, 893 N Warson Rd, St Louis, MO 63141 USA..(2001).Stretching single-stranded DNA: Interplay of electrostatic, base-pairing, and base-pair stacking interactions.BIOPHYSICAL JOURNAL,81(2),1133-1143.
MLA Ou-Yang, ZC,et al."Stretching single-stranded DNA: Interplay of electrostatic, base-pairing, and base-pair stacking interactions".BIOPHYSICAL JOURNAL 81.2(2001):1133-1143.
Files in This Item:
File Name/Size DocType Version Access License
Stretching Single-St(546KB) 开放获取LicenseApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Ou-Yang, ZC]'s Articles
[Zhou, HJ]'s Articles
[Zhang, Y , Donald Danforth Plant Sci Ctr, Lab Comp Genom, 893 N Warson Rd, St Louis, MO 63141 USA.]'s Articles
Baidu academic
Similar articles in Baidu academic
[Ou-Yang, ZC]'s Articles
[Zhou, HJ]'s Articles
[Zhang, Y , Donald Danforth Plant Sci Ctr, Lab Comp Genom, 893 N Warson Rd, St Louis, MO 63141 USA.]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Ou-Yang, ZC]'s Articles
[Zhou, HJ]'s Articles
[Zhang, Y , Donald Danforth Plant Sci Ctr, Lab Comp Genom, 893 N Warson Rd, St Louis, MO 63141 USA.]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.