Liu, F , Tsing Hua Univ, Ctr Adv Study, Beijing 100084, Peoples R China.
部门归属:
Tsing Hua Univ, Ctr Adv Study, Beijing 100084, Peoples R China; Chinese Acad Sci, Inst Theoret Phys, Beijing 100080, Peoples R China
英文摘要:
Using simple polymer elastic theory and known RNA free energies, we study the single RNA folding and unfolding on the secondary structure level under mechanical constant force by stochastic kinetic simulation. As a primary application, this method is used to simulate the experiment performed by Liphardt et al. [Science 292, 733 (2001)]. The extension-force curves in equilibrium and kinetic reaction rate constants for folding and unfolding are calculated. Our results show that the agreement between simulation and experimental measurements is satisfactory.
