ITP OpenIR  > 理论物理所科研产出  > SCI论文
Hao, Y; Xu, LF; Shi, HL; Shi, HL (reprint author), Chinese Acad Sci, Inst Theoret Phys, Beijing 100190, Peoples R China.
Theoretical Analysis of Catalytic-sRNA-Mediated Gene Silencing
Source PublicationJOURNAL OF MOLECULAR BIOLOGY
Language英语
KeywordTranscriptional Regulation Escherichia-coli Regulatory Rnas Bacteria Expression Noise Consequences Numbers Stress System
AbstractSmall regulatory RNA (sRNA) that acts by an antisense mechanism is critical for gene regulation at the posttranscriptional level. Recently, an Hfq-dependent sRNA named MicM, which is related to the regulation of outer membrane protein, was verified as a novel antisense sRNA due to its catalytic mode of regulation. Here we propose a simple kinetic model for the enzyme-like regulation mode of sRNA and study in detail the noise properties of the target gene under various recycling rates of the regulator. We predict that the recycling rate of sRNA and other relative parameters have significant influence on the noise strength of target expression. In comparison with the stoichiometric regulatory mode, a lesser fluctuation of target expression was observed near the threshold at which the transcription rates of both sRNA and target mRNA equal each other. We also found that the new mode is better in terms of rapid response to external signals. However, it needs more time to achieve target recovery if the stimulating signal disappears. Additionally, the obtained time evolution results of the MicM-ybfM interaction system based on our model are consistent with previous experimental results, serving as experimental evidence to back up our theoretical analysis. (C) 2010 Elsevier Ltd. All rights reserved.
2011
ISSN0022-2836
Volume406Issue:1Pages:195-204
Subject AreaPhysics
Indexed BySCI
Funding OrganizationNational Basic Research Program of China [2007CB814800] ; National Basic Research Program of China [2007CB814800] ; National Basic Research Program of China [2007CB814800] ; National Basic Research Program of China [2007CB814800]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.itp.ac.cn/handle/311006/14411
Collection理论物理所科研产出_SCI论文
Corresponding AuthorShi, HL (reprint author), Chinese Acad Sci, Inst Theoret Phys, Beijing 100190, Peoples R China.
Recommended Citation
GB/T 7714
Hao, Y,Xu, LF,Shi, HL,et al. Theoretical Analysis of Catalytic-sRNA-Mediated Gene Silencing[J]. JOURNAL OF MOLECULAR BIOLOGY,2011,406(1):195-204.
APA Hao, Y,Xu, LF,Shi, HL,&Shi, HL .(2011).Theoretical Analysis of Catalytic-sRNA-Mediated Gene Silencing.JOURNAL OF MOLECULAR BIOLOGY,406(1),195-204.
MLA Hao, Y,et al."Theoretical Analysis of Catalytic-sRNA-Mediated Gene Silencing".JOURNAL OF MOLECULAR BIOLOGY 406.1(2011):195-204.
Files in This Item:
File Name/Size DocType Version Access License
Theoretical Analysis(352KB) 开放获取LicenseApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Hao, Y]'s Articles
[Xu, LF]'s Articles
[Shi, HL]'s Articles
Baidu academic
Similar articles in Baidu academic
[Hao, Y]'s Articles
[Xu, LF]'s Articles
[Shi, HL]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Hao, Y]'s Articles
[Xu, LF]'s Articles
[Shi, HL]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.