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题名: Theoretical Analysis of Catalytic-sRNA-Mediated Gene Silencing
作者: Hao, Y ;  Xu, LF ;  Shi, HL
刊名: JOURNAL OF MOLECULAR BIOLOGY
出版日期: 2011
卷号: 406, 期号:1, 页码:195-204
关键词: TRANSCRIPTIONAL REGULATION ;  ESCHERICHIA-COLI ;  REGULATORY RNAS ;  BACTERIA ;  EXPRESSION ;  NOISE ;  CONSEQUENCES ;  NUMBERS ;  STRESS ;  SYSTEM
学科分类: Physics
通讯作者: Shi, HL (reprint author), Chinese Acad Sci, Inst Theoret Phys, Beijing 100190, Peoples R China.
部门归属: [Hao, Yue; Xu, Liufang; Shi, Hualin] Chinese Acad Sci, Inst Theoret Phys, Beijing 100190, Peoples R China
英文摘要: Small regulatory RNA (sRNA) that acts by an antisense mechanism is critical for gene regulation at the posttranscriptional level. Recently, an Hfq-dependent sRNA named MicM, which is related to the regulation of outer membrane protein, was verified as a novel antisense sRNA due to its catalytic mode of regulation. Here we propose a simple kinetic model for the enzyme-like regulation mode of sRNA and study in detail the noise properties of the target gene under various recycling rates of the regulator. We predict that the recycling rate of sRNA and other relative parameters have significant influence on the noise strength of target expression. In comparison with the stoichiometric regulatory mode, a lesser fluctuation of target expression was observed near the threshold at which the transcription rates of both sRNA and target mRNA equal each other. We also found that the new mode is better in terms of rapid response to external signals. However, it needs more time to achieve target recovery if the stimulating signal disappears. Additionally, the obtained time evolution results of the MicM-ybfM interaction system based on our model are consistent with previous experimental results, serving as experimental evidence to back up our theoretical analysis. (C) 2010 Elsevier Ltd. All rights reserved.
资助者: National Basic Research Program of China [2007CB814800]
收录类别: SCI
原文出处: 查看原文
语种: 英语
WOS记录号: WOS:000287281800015
Citation statistics: 
内容类型: 期刊论文
URI标识: http://ir.itp.ac.cn/handle/311006/14411
Appears in Collections:理论物理所2011年知识产出_期刊论文

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Recommended Citation:
Hao, Y,Xu, LF,Shi, HL. Theoretical Analysis of Catalytic-sRNA-Mediated Gene Silencing[J]. JOURNAL OF MOLECULAR BIOLOGY,2011,406(1):195-204.
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