ITP OpenIR  > 理论物理所2014年知识产出
Sub-nanometer drift correction for super-resolution imaging
Tang, Y; Wang, X; Zhang, X; Li, J; Dai, L; Tang, Y (reprint author), Chongqing Univ, Dept Phys, Chongqing 400044, Peoples R China.
2014
发表期刊OPTICS LETTERS
ISSN0146-9592
卷号39期号:19页码:5685-5688
摘要Spatial resolution of conventional far-field fluorescence microscopy is limited by diffraction of light. Single-molecule localization microscopy (SMLM), such as (direct) stochastic optical reconstruction microscopy (dSTORM/STORM), and (fluorescence) photoactivation localization microscopy (fPALM/PALM), can break this barrier by localizing single emitters and reconstructing super-resolution image with much higher precision. Nevertheless, a SMLM measurement needs to record a large number of image frames and takes considerable recording time. In this process, sample drift becomes a critical problem and cannot be neglected. In this Letter, we present a sub-nanometer precision, low-cost sample drift correction method based on minimizing normalized root-mean-square error (NRMSE) between bright field images. Two optical configurations are suggested for recording bright field and fluorescence images simultaneously or alternately. The method was demonstrated on simulated data, and better than 0.3 nm drift correction precision was achieved. It was also applied on dSTORM imaging of F-actins of 3T3 cell, and the quality of reconstructed super-resolution image was improved observably. This method does not require special hardware, extra labelling or markers, and no precision decline due to photobleaching. It can be applied as an add-on for SMLM setups and achieves sub-nanometer precision drift correction for post-measurement or real time drift compensation. (C) 2014 Optical Society of America
部门归属[Tang, Y.] Chongqing Univ, Dept Phys, Chongqing 400044, Peoples R China ; [Tang, Y. ; Zhang, X. ; Li, J. ; Dai, L.] Natl Ctr Nanosci & Technol China, Beijing 100190, Peoples R China ; [Tang, Y. ; Wang, X.] Chinese Acad Sci, Inst Theoret Phys, State Key Lab Theoret Phys, Beijing 100190, Peoples R China ; [Li, J.] Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China
关键词Photoactivation Localization Microscopy Optical Microscope Fluorescence Reconstruction Registration Nm
学科领域Physics
资助者National Natural Science Foundation of China [21273053]; National Basic Research Program of China (973 program) [2013CB932804]; Major Research Plan of the National Natural Science Foundation of China [91027045] ; National Natural Science Foundation of China [21273053]; National Basic Research Program of China (973 program) [2013CB932804]; Major Research Plan of the National Natural Science Foundation of China [91027045] ; National Natural Science Foundation of China [21273053]; National Basic Research Program of China (973 program) [2013CB932804]; Major Research Plan of the National Natural Science Foundation of China [91027045] ; National Natural Science Foundation of China [21273053]; National Basic Research Program of China (973 program) [2013CB932804]; Major Research Plan of the National Natural Science Foundation of China [91027045]
DOI10.1364/OL.39.005685
URL查看原文
收录类别SCI
语种英语
资助者National Natural Science Foundation of China [21273053]; National Basic Research Program of China (973 program) [2013CB932804]; Major Research Plan of the National Natural Science Foundation of China [91027045] ; National Natural Science Foundation of China [21273053]; National Basic Research Program of China (973 program) [2013CB932804]; Major Research Plan of the National Natural Science Foundation of China [91027045] ; National Natural Science Foundation of China [21273053]; National Basic Research Program of China (973 program) [2013CB932804]; Major Research Plan of the National Natural Science Foundation of China [91027045] ; National Natural Science Foundation of China [21273053]; National Basic Research Program of China (973 program) [2013CB932804]; Major Research Plan of the National Natural Science Foundation of China [91027045]
WOS记录号WOS:000343906400055
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.itp.ac.cn/handle/311006/15643
专题理论物理所2014年知识产出
通讯作者Tang, Y (reprint author), Chongqing Univ, Dept Phys, Chongqing 400044, Peoples R China.
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GB/T 7714
Tang, Y,Wang, X,Zhang, X,et al. Sub-nanometer drift correction for super-resolution imaging[J]. OPTICS LETTERS,2014,39(19):5685-5688.
APA Tang, Y,Wang, X,Zhang, X,Li, J,Dai, L,&Tang, Y .(2014).Sub-nanometer drift correction for super-resolution imaging.OPTICS LETTERS,39(19),5685-5688.
MLA Tang, Y,et al."Sub-nanometer drift correction for super-resolution imaging".OPTICS LETTERS 39.19(2014):5685-5688.
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