Knowledge Management System of Institute of Theoretical Physics, CAS
Xu, Y; Chen, H; Qu, YJ; Efremov, AK; Li, M; Ouyang, ZC; Liu, DS; Yan, J; Liu, DS (reprint author), Tsinghua Univ, Minist Educ, Dept Chem, Key Lab Organ Optoelect & Mol Engn, Beijing 100084, Peoples R China. | |
Mechano-chemical selections of two competitive unfolding pathways of a single DNA i-motif | |
发表期刊 | CHINESE PHYSICS B |
语种 | 英语 |
关键词 | G-quadruplex Mechanical Stability Rna-polymerase Stranded-dna Double Helix B-dna Molecule Force Proteins Dynamics |
摘要 | The DNA i-motif is a quadruplex structure formed in tandem cytosine-rich sequences in slightly acidic conditions. Besides being considered as a building block of DNA nano-devices, it may also play potential roles in regulating chromosome stability and gene transcriptions. The stability of i-motif is crucial for these functions. In this work, we investigated the mechanical stability of a single i-motif formed in the human telomeric sequence 5'-(CCCTAA)(3)CCC, which revealed a novel pH and loading rate-dependent bimodal unfolding force distribution. Although the cause of the bimodal unfolding force species is not clear, we proposed a phenomenological model involving a direct unfolding favored at lower loading rate or higher pH value, which is subject to competition with another unfolding pathway through a mechanically stable intermediate state whose nature is yet to be determined. Overall, the unique mechano-chemical responses of i-motif-provide a new perspective to its stability, which may be useful to guide designing new i-motif-based DNA mechanical nano-devices. |
2014 | |
ISSN | 1674-1056 |
卷号 | 23期号:6页码:68702 |
学科领域 | Physics |
DOI | 10.1088/1674-1056/23/6/068702 |
收录类别 | SCI |
项目资助者 | National Research Foundation through the Mechanobiology Institute Singapore and the Ministry of Education of Singapore [MOE2012-T3-1-001]; National Basic Research Program of China [2013CB932800]; Major Research Plan of the National Natural Science Foundation of China [91027046, 91027045]; Fundamental Research Funds for the Central Universities [2013121005] ; National Research Foundation through the Mechanobiology Institute Singapore and the Ministry of Education of Singapore [MOE2012-T3-1-001]; National Basic Research Program of China [2013CB932800]; Major Research Plan of the National Natural Science Foundation of China [91027046, 91027045]; Fundamental Research Funds for the Central Universities [2013121005] ; National Research Foundation through the Mechanobiology Institute Singapore and the Ministry of Education of Singapore [MOE2012-T3-1-001]; National Basic Research Program of China [2013CB932800]; Major Research Plan of the National Natural Science Foundation of China [91027046, 91027045]; Fundamental Research Funds for the Central Universities [2013121005] ; National Research Foundation through the Mechanobiology Institute Singapore and the Ministry of Education of Singapore [MOE2012-T3-1-001]; National Basic Research Program of China [2013CB932800]; Major Research Plan of the National Natural Science Foundation of China [91027046, 91027045]; Fundamental Research Funds for the Central Universities [2013121005] |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.itp.ac.cn/handle/311006/15749 |
专题 | SCI期刊论文 |
通讯作者 | Liu, DS (reprint author), Tsinghua Univ, Minist Educ, Dept Chem, Key Lab Organ Optoelect & Mol Engn, Beijing 100084, Peoples R China. |
推荐引用方式 GB/T 7714 | Xu, Y,Chen, H,Qu, YJ,et al. Mechano-chemical selections of two competitive unfolding pathways of a single DNA i-motif[J]. CHINESE PHYSICS B,2014,23(6):68702. |
APA | Xu, Y.,Chen, H.,Qu, YJ.,Efremov, AK.,Li, M.,...&Liu, DS .(2014).Mechano-chemical selections of two competitive unfolding pathways of a single DNA i-motif.CHINESE PHYSICS B,23(6),68702. |
MLA | Xu, Y,et al."Mechano-chemical selections of two competitive unfolding pathways of a single DNA i-motif".CHINESE PHYSICS B 23.6(2014):68702. |
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