ITP OpenIR  > 理论物理所2015年知识产出
SNSMIL, a real-time single molecule identification and localization algorithm for super-resolution fluorescence microscopy
Tang, YQ; Dai, LR; Zhang, XM; Li, JB; Hendriks, JN; Fan, XM; Gruteser, N; Meisenberg, A; Baumann, A; Katranidis, A; Gensch, T; Li, JB (reprint author), Natl Ctr Nanosci & Technol China, 11 Beiyitiao Zhongguancun, Beijing 100190, Peoples R China.
2015
发表期刊SCIENTIFIC REPORTS
卷号5期号:0页码:11073
文章类型Article
摘要Single molecule localization based super-resolution fluorescence microscopy offers significantly higher spatial resolution than predicted by Abbe's resolution limit for far field optical microscopy. Such super-resolution images are reconstructed from wide-field or total internal reflection single molecule fluorescence recordings. Discrimination between emission of single fluorescent molecules and background noise fluctuations remains a great challenge in current data analysis. Here we present a real-time, and robust single molecule identification and localization algorithm, SNSMIL (Shot Noise based Single Molecule Identification and Localization). This algorithm is based on the intrinsic nature of noise, i.e., its Poisson or shot noise characteristics and a new identification criterion, Q(SNSMIL), is defined. SNSMIL improves the identification accuracy of single fluorescent molecules in experimental or simulated datasets with high and inhomogeneous background. The implementation of SNSMIL relies on a graphics processing unit (GPU), making real-time analysis feasible as shown for real experimental and simulated datasets.
学科领域Science & Technology - Other Topics
资助者National Natural Science Foundation of China [21273053, 21433010, 21320102004] ; National Natural Science Foundation of China [21273053, 21433010, 21320102004] ; Instrument Developing Project of the Chinese Academy of Sciences [YZ201417] ; Instrument Developing Project of the Chinese Academy of Sciences [YZ201417] ; China Scholarship Council ; China Scholarship Council ; National Natural Science Foundation of China [21273053, 21433010, 21320102004] ; National Natural Science Foundation of China [21273053, 21433010, 21320102004] ; Instrument Developing Project of the Chinese Academy of Sciences [YZ201417] ; Instrument Developing Project of the Chinese Academy of Sciences [YZ201417] ; China Scholarship Council ; China Scholarship Council
DOIhttp://dx.doi.org/10.1038/srep11073
关键词[WOS]RESOLUTION LIMIT ; IMAGE-ANALYSIS ; NANOSCOPY ; DEPLETION ; SOFTWARE ; TRACKING ; REVEALS ; SYSTEMS ; ACTIN ; STORM
收录类别SCI
语种英语
资助者National Natural Science Foundation of China [21273053, 21433010, 21320102004] ; National Natural Science Foundation of China [21273053, 21433010, 21320102004] ; Instrument Developing Project of the Chinese Academy of Sciences [YZ201417] ; Instrument Developing Project of the Chinese Academy of Sciences [YZ201417] ; China Scholarship Council ; China Scholarship Council ; National Natural Science Foundation of China [21273053, 21433010, 21320102004] ; National Natural Science Foundation of China [21273053, 21433010, 21320102004] ; Instrument Developing Project of the Chinese Academy of Sciences [YZ201417] ; Instrument Developing Project of the Chinese Academy of Sciences [YZ201417] ; China Scholarship Council ; China Scholarship Council
WOS类目Multidisciplinary Sciences
引用统计
文献类型期刊论文
条目标识符http://ir.itp.ac.cn/handle/311006/20957
专题理论物理所2015年知识产出
通讯作者Li, JB (reprint author), Natl Ctr Nanosci & Technol China, 11 Beiyitiao Zhongguancun, Beijing 100190, Peoples R China.
推荐引用方式
GB/T 7714
Tang, YQ,Dai, LR,Zhang, XM,et al. SNSMIL, a real-time single molecule identification and localization algorithm for super-resolution fluorescence microscopy[J]. SCIENTIFIC REPORTS,2015,5(0):11073.
APA Tang, YQ.,Dai, LR.,Zhang, XM.,Li, JB.,Hendriks, JN.,...&Li, JB .(2015).SNSMIL, a real-time single molecule identification and localization algorithm for super-resolution fluorescence microscopy.SCIENTIFIC REPORTS,5(0),11073.
MLA Tang, YQ,et al."SNSMIL, a real-time single molecule identification and localization algorithm for super-resolution fluorescence microscopy".SCIENTIFIC REPORTS 5.0(2015):11073.
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