ITP OpenIR  > 理论物理所科研产出  > SCI论文
Tang, YQ; Dai, LR; Zhang, XM; Li, JB; Hendriks, JN; Fan, XM; Gruteser, N; Meisenberg, A; Baumann, A; Katranidis, A; Gensch, T; Li, JB (reprint author), Natl Ctr Nanosci & Technol China, 11 Beiyitiao Zhongguancun, Beijing 100190, Peoples R China.
SNSMIL, a real-time single molecule identification and localization algorithm for super-resolution fluorescence microscopy
Source PublicationSCIENTIFIC REPORTS
Language英语
AbstractSingle molecule localization based super-resolution fluorescence microscopy offers significantly higher spatial resolution than predicted by Abbe's resolution limit for far field optical microscopy. Such super-resolution images are reconstructed from wide-field or total internal reflection single molecule fluorescence recordings. Discrimination between emission of single fluorescent molecules and background noise fluctuations remains a great challenge in current data analysis. Here we present a real-time, and robust single molecule identification and localization algorithm, SNSMIL (Shot Noise based Single Molecule Identification and Localization). This algorithm is based on the intrinsic nature of noise, i.e., its Poisson or shot noise characteristics and a new identification criterion, Q(SNSMIL), is defined. SNSMIL improves the identification accuracy of single fluorescent molecules in experimental or simulated datasets with high and inhomogeneous background. The implementation of SNSMIL relies on a graphics processing unit (GPU), making real-time analysis feasible as shown for real experimental and simulated datasets.
2015
Volume5Issue:0Pages:11073
Subject AreaScience & Technology - Other Topics
DOIhttp://dx.doi.org/10.1038/srep11073
Indexed BySCI
Funding OrganizationNational Natural Science Foundation of China [21273053, 21433010, 21320102004] ; National Natural Science Foundation of China [21273053, 21433010, 21320102004] ; National Natural Science Foundation of China [21273053, 21433010, 21320102004] ; National Natural Science Foundation of China [21273053, 21433010, 21320102004] ; Instrument Developing Project of the Chinese Academy of Sciences [YZ201417] ; Instrument Developing Project of the Chinese Academy of Sciences [YZ201417] ; Instrument Developing Project of the Chinese Academy of Sciences [YZ201417] ; Instrument Developing Project of the Chinese Academy of Sciences [YZ201417] ; China Scholarship Council ; China Scholarship Council ; China Scholarship Council ; China Scholarship Council
Citation statistics
Document Type期刊论文
Identifierhttp://ir.itp.ac.cn/handle/311006/20957
Collection理论物理所科研产出_SCI论文
Corresponding AuthorLi, JB (reprint author), Natl Ctr Nanosci & Technol China, 11 Beiyitiao Zhongguancun, Beijing 100190, Peoples R China.
Recommended Citation
GB/T 7714
Tang, YQ,Dai, LR,Zhang, XM,et al. SNSMIL, a real-time single molecule identification and localization algorithm for super-resolution fluorescence microscopy[J]. SCIENTIFIC REPORTS,2015,5(0):11073.
APA Tang, YQ.,Dai, LR.,Zhang, XM.,Li, JB.,Hendriks, JN.,...&Li, JB .(2015).SNSMIL, a real-time single molecule identification and localization algorithm for super-resolution fluorescence microscopy.SCIENTIFIC REPORTS,5(0),11073.
MLA Tang, YQ,et al."SNSMIL, a real-time single molecule identification and localization algorithm for super-resolution fluorescence microscopy".SCIENTIFIC REPORTS 5.0(2015):11073.
Files in This Item:
File Name/Size DocType Version Access License
SNSMIL, a real-time (1768KB)期刊论文出版稿开放获取CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Tang, YQ]'s Articles
[Dai, LR]'s Articles
[Zhang, XM]'s Articles
Baidu academic
Similar articles in Baidu academic
[Tang, YQ]'s Articles
[Dai, LR]'s Articles
[Zhang, XM]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Tang, YQ]'s Articles
[Dai, LR]'s Articles
[Zhang, XM]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.