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题名: Effects of cooperation between translating ribosome and RNA polymerase on termination efficiency of the Rho-independent terminator
作者: Li, R ;  Zhang, Q ;  Li, JB ;  Shi, HL
刊名: NUCLEIC ACIDS RESEARCH
出版日期: 2016
卷号: 44, 期号:6, 页码:2554-2563
学科分类: Biochemistry & Molecular Biology
DOI: http://dx.doi.org/10.1093/nar/gkv1285
通讯作者: Shi, HL (reprint author), Chinese Acad Sci, Inst Theoret Phys, State Key Lab Theoret Phys, Beijing 100190, Peoples R China. ;  Li, JB (reprint author), Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China. ;  Li, JB (reprint author), Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China.
文章类型: Article
英文摘要: An experimental system was designed to measure in vivo termination efficiency (TE) of the Rho-independent terminator and position-function relations were quantified for the terminator tR2 in Escherichia coli. The terminator function was almost completely repressed when tR2 was located several base pairs downstream from the gene, and TE gradually increased to maximum values with the increasing distance between the gene and terminator. This TE-distance relation reflected a stochastic coupling of the ribosome and RNA polymerase (RNAP). Terminators located in the first 100 bp of the coding region can function efficiently. However, functional repression was observed when the terminator was located in the latter part of the coding region, and the degree of repression was determined by transcriptional and translational dynamics. These results may help to elucidate mechanisms of Rho-independent termination and reveal genomic locations of terminators and functions of the sequence that precedes terminators. These observations may have important applications in synthetic biology.
类目[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: INTRINSIC TRANSCRIPTION TERMINATION ;  GENOME-WIDE ANALYSIS ;  ESCHERICHIA-COLI ;  IN-VIVO ;  BACTERIOPHAGE-LAMBDA ;  GENE-EXPRESSION ;  NASCENT RNA ;  ELONGATION ;  ATTENUATOR ;  PREDICTION
收录类别: SCI
项目资助者: National Basic research Program of China [2013CB834100] ;  National Natural Science Foundation of China [11121403, 11274320]
语种: 英语
Citation statistics: 
内容类型: 期刊论文
URI标识: http://ir.itp.ac.cn/handle/311006/21700
Appears in Collections:理论物理所2016年知识产出_期刊论文

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Recommended Citation:
Li, R,Zhang, Q,Li, JB,et al. Effects of cooperation between translating ribosome and RNA polymerase on termination efficiency of the Rho-independent terminator[J]. NUCLEIC ACIDS RESEARCH,2016,44(6):2554-2563.
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