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Effects of cooperation between translating ribosome and RNA polymerase on termination efficiency of the Rho-independent terminator
Li, R; Zhang, Q; Li, JB; Shi, HL; Shi, HL (reprint author), Chinese Acad Sci, Inst Theoret Phys, State Key Lab Theoret Phys, Beijing 100190, Peoples R China.; Li, JB (reprint author), Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China.; Li, JB (reprint author), Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China.
2016
发表期刊NUCLEIC ACIDS RESEARCH
卷号44期号:6页码:2554-2563
文章类型Article
摘要An experimental system was designed to measure in vivo termination efficiency (TE) of the Rho-independent terminator and position-function relations were quantified for the terminator tR2 in Escherichia coli. The terminator function was almost completely repressed when tR2 was located several base pairs downstream from the gene, and TE gradually increased to maximum values with the increasing distance between the gene and terminator. This TE-distance relation reflected a stochastic coupling of the ribosome and RNA polymerase (RNAP). Terminators located in the first 100 bp of the coding region can function efficiently. However, functional repression was observed when the terminator was located in the latter part of the coding region, and the degree of repression was determined by transcriptional and translational dynamics. These results may help to elucidate mechanisms of Rho-independent termination and reveal genomic locations of terminators and functions of the sequence that precedes terminators. These observations may have important applications in synthetic biology.
学科领域Biochemistry & Molecular Biology
资助者National Basic research Program of China [2013CB834100] ; National Basic research Program of China [2013CB834100] ; National Basic research Program of China [2013CB834100] ; National Basic research Program of China [2013CB834100] ; National Natural Science Foundation of China [11121403, 11274320] ; National Natural Science Foundation of China [11121403, 11274320] ; National Natural Science Foundation of China [11121403, 11274320] ; National Natural Science Foundation of China [11121403, 11274320] ; National Basic research Program of China [2013CB834100] ; National Basic research Program of China [2013CB834100] ; National Basic research Program of China [2013CB834100] ; National Basic research Program of China [2013CB834100] ; National Natural Science Foundation of China [11121403, 11274320] ; National Natural Science Foundation of China [11121403, 11274320] ; National Natural Science Foundation of China [11121403, 11274320] ; National Natural Science Foundation of China [11121403, 11274320]
DOIhttp://dx.doi.org/10.1093/nar/gkv1285
关键词[WOS]INTRINSIC TRANSCRIPTION TERMINATION ; GENOME-WIDE ANALYSIS ; ESCHERICHIA-COLI ; IN-VIVO ; BACTERIOPHAGE-LAMBDA ; GENE-EXPRESSION ; NASCENT RNA ; ELONGATION ; ATTENUATOR ; PREDICTION
收录类别SCI
语种英语
资助者National Basic research Program of China [2013CB834100] ; National Basic research Program of China [2013CB834100] ; National Basic research Program of China [2013CB834100] ; National Basic research Program of China [2013CB834100] ; National Natural Science Foundation of China [11121403, 11274320] ; National Natural Science Foundation of China [11121403, 11274320] ; National Natural Science Foundation of China [11121403, 11274320] ; National Natural Science Foundation of China [11121403, 11274320] ; National Basic research Program of China [2013CB834100] ; National Basic research Program of China [2013CB834100] ; National Basic research Program of China [2013CB834100] ; National Basic research Program of China [2013CB834100] ; National Natural Science Foundation of China [11121403, 11274320] ; National Natural Science Foundation of China [11121403, 11274320] ; National Natural Science Foundation of China [11121403, 11274320] ; National Natural Science Foundation of China [11121403, 11274320]
WOS类目Biochemistry & Molecular Biology
引用统计
文献类型期刊论文
条目标识符http://ir.itp.ac.cn/handle/311006/21700
专题理论物理所SCI论文
通讯作者Shi, HL (reprint author), Chinese Acad Sci, Inst Theoret Phys, State Key Lab Theoret Phys, Beijing 100190, Peoples R China.; Li, JB (reprint author), Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China.; Li, JB (reprint author), Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China.
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Li, R,Zhang, Q,Li, JB,et al. Effects of cooperation between translating ribosome and RNA polymerase on termination efficiency of the Rho-independent terminator[J]. NUCLEIC ACIDS RESEARCH,2016,44(6):2554-2563.
APA Li, R.,Zhang, Q.,Li, JB.,Shi, HL.,Shi, HL .,...&Li, JB .(2016).Effects of cooperation between translating ribosome and RNA polymerase on termination efficiency of the Rho-independent terminator.NUCLEIC ACIDS RESEARCH,44(6),2554-2563.
MLA Li, R,et al."Effects of cooperation between translating ribosome and RNA polymerase on termination efficiency of the Rho-independent terminator".NUCLEIC ACIDS RESEARCH 44.6(2016):2554-2563.
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