ITP OpenIR  > 理论物理所2017年知识产出
Anchor Effect of Interactions Between Kinesin's Nucleotide-Binding Pocket and Microtubule
Jin, YM; Geng, YZ; Lu, LN; Ma, YL; Lu, G; Zhang, H; Ji, Q; Geng, YZ (reprint author), Hebei Univ Technol, Sch Sci, Tianjin 300401, Peoples R China.; Ji, Q (reprint author), Chinese Acad Sci, Inst Theoret Phys, State Key Lab Theoret Phys, Beijing 100190, Peoples R China.
2017
发表期刊CELLULAR AND MOLECULAR BIOENGINEERING
卷号10期号:2页码:162-173
文章类型Article
摘要Microtubule not only provides the track for kinesin but also modulates kinesin's mechanochemical cycle. Microtubule binding greatly increases the rates of two chemical steps occurring inside the nucleotide-binding pocket (NBP) of kinesin, i.e., ATP hydrolysis and ADP release. Kinesin neck linker docking (the key force-generation step) is initiated by the motor head rotation induced by ATP binding which needs an anchor provided by microtubule. These functions of microtubule can only be accomplished through interactions with kinesin. Based on the newly obtained crystal structures of kinesin-microtubule complexes, we investigate the interactions between kinesin's NBP and microtubule using molecular dynamics simulations. We find that the N-3 motif of NBP has direct interactions with a group of negatively charged residues on alpha-tubulin through Ser235 and Lys237. These specific long-range interactions induce binding of NBP to microtubule at the right position and assist the formation of the indirect interaction between NBP and microtubule. These interactions between N-3 and microtubule have an important anchor effect for kinesin's motor domain during its rotation with Ser235 as the rotation center, and also play a crucial role in stabilizing the ATP-hydrolysis environment.
关键词Kinesin Microtubule Nucleotide-binding Pocket Anchor Effect
学科领域Cell Biology ; Biophysics
资助者National Natural Science Foundation of China [11545014, 11605038] ; National Natural Science Foundation of China [11545014, 11605038] ; Open Project Program of State Key Laboratory of Theoretical Physics, Institute of Theoretical Physics, Chinese Academy of Science, China [Y5KF211CJ1] ; Open Project Program of State Key Laboratory of Theoretical Physics, Institute of Theoretical Physics, Chinese Academy of Science, China [Y5KF211CJ1] ; National Natural Science Foundation of China [11545014, 11605038] ; National Natural Science Foundation of China [11545014, 11605038] ; Open Project Program of State Key Laboratory of Theoretical Physics, Institute of Theoretical Physics, Chinese Academy of Science, China [Y5KF211CJ1] ; Open Project Program of State Key Laboratory of Theoretical Physics, Institute of Theoretical Physics, Chinese Academy of Science, China [Y5KF211CJ1]
DOIhttp://dx.doi.org/10.1007/s12195-017-0477-8
关键词[WOS]MOLECULAR-DYNAMICS ; MOTOR DOMAIN ; INTRACELLULAR-TRANSPORT ; CRYSTAL-STRUCTURE ; EPR SPECTROSCOPY ; PROTEINS ; MECHANISM ; TUBULIN ; HYDROLYSIS ; MYOSIN
语种英语
资助者National Natural Science Foundation of China [11545014, 11605038] ; National Natural Science Foundation of China [11545014, 11605038] ; Open Project Program of State Key Laboratory of Theoretical Physics, Institute of Theoretical Physics, Chinese Academy of Science, China [Y5KF211CJ1] ; Open Project Program of State Key Laboratory of Theoretical Physics, Institute of Theoretical Physics, Chinese Academy of Science, China [Y5KF211CJ1] ; National Natural Science Foundation of China [11545014, 11605038] ; National Natural Science Foundation of China [11545014, 11605038] ; Open Project Program of State Key Laboratory of Theoretical Physics, Institute of Theoretical Physics, Chinese Academy of Science, China [Y5KF211CJ1] ; Open Project Program of State Key Laboratory of Theoretical Physics, Institute of Theoretical Physics, Chinese Academy of Science, China [Y5KF211CJ1]
WOS类目Cell & Tissue Engineering ; Biophysics ; Cell Biology
引用统计
文献类型期刊论文
条目标识符http://ir.itp.ac.cn/handle/311006/22094
专题理论物理所2017年知识产出
通讯作者Geng, YZ (reprint author), Hebei Univ Technol, Sch Sci, Tianjin 300401, Peoples R China.; Ji, Q (reprint author), Chinese Acad Sci, Inst Theoret Phys, State Key Lab Theoret Phys, Beijing 100190, Peoples R China.
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Jin, YM,Geng, YZ,Lu, LN,et al. Anchor Effect of Interactions Between Kinesin's Nucleotide-Binding Pocket and Microtubule[J]. CELLULAR AND MOLECULAR BIOENGINEERING,2017,10(2):162-173.
APA Jin, YM.,Geng, YZ.,Lu, LN.,Ma, YL.,Lu, G.,...&Ji, Q .(2017).Anchor Effect of Interactions Between Kinesin's Nucleotide-Binding Pocket and Microtubule.CELLULAR AND MOLECULAR BIOENGINEERING,10(2),162-173.
MLA Jin, YM,et al."Anchor Effect of Interactions Between Kinesin's Nucleotide-Binding Pocket and Microtubule".CELLULAR AND MOLECULAR BIOENGINEERING 10.2(2017):162-173.
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