Knowledge Management System of Institute of Theoretical Physics, CAS
| Jin, YM; Geng, YZ; Lu, LN; Ma, YL; Lu, G; Zhang, H; Ji, Q; Geng, YZ (reprint author), Hebei Univ Technol, Sch Sci, Tianjin 300401, Peoples R China.; Ji, Q (reprint author), Chinese Acad Sci, Inst Theoret Phys, State Key Lab Theoret Phys, Beijing 100190, Peoples R China. | |
| Anchor Effect of Interactions Between Kinesin's Nucleotide-Binding Pocket and Microtubule | |
| Source Publication | CELLULAR AND MOLECULAR BIOENGINEERING
 |
| Language | 英语 |
| Keyword | Kinesin Microtubule Nucleotide-binding Pocket Anchor Effect |
| Abstract | Microtubule not only provides the track for kinesin but also modulates kinesin's mechanochemical cycle. Microtubule binding greatly increases the rates of two chemical steps occurring inside the nucleotide-binding pocket (NBP) of kinesin, i.e., ATP hydrolysis and ADP release. Kinesin neck linker docking (the key force-generation step) is initiated by the motor head rotation induced by ATP binding which needs an anchor provided by microtubule. These functions of microtubule can only be accomplished through interactions with kinesin. Based on the newly obtained crystal structures of kinesin-microtubule complexes, we investigate the interactions between kinesin's NBP and microtubule using molecular dynamics simulations. We find that the N-3 motif of NBP has direct interactions with a group of negatively charged residues on alpha-tubulin through Ser235 and Lys237. These specific long-range interactions induce binding of NBP to microtubule at the right position and assist the formation of the indirect interaction between NBP and microtubule. These interactions between N-3 and microtubule have an important anchor effect for kinesin's motor domain during its rotation with Ser235 as the rotation center, and also play a crucial role in stabilizing the ATP-hydrolysis environment. |
| 2017 | |
| Volume | 10Issue:2Pages:162-173 |
| Subject Area | Cell Biology ; Biophysics |
| DOI | http://dx.doi.org/10.1007/s12195-017-0477-8 |
| Funding Organization | National Natural Science Foundation of China [11545014, 11605038] ; National Natural Science Foundation of China [11545014, 11605038] ; National Natural Science Foundation of China [11545014, 11605038] ; National Natural Science Foundation of China [11545014, 11605038] ; Open Project Program of State Key Laboratory of Theoretical Physics, Institute of Theoretical Physics, Chinese Academy of Science, China [Y5KF211CJ1] ; Open Project Program of State Key Laboratory of Theoretical Physics, Institute of Theoretical Physics, Chinese Academy of Science, China [Y5KF211CJ1] ; Open Project Program of State Key Laboratory of Theoretical Physics, Institute of Theoretical Physics, Chinese Academy of Science, China [Y5KF211CJ1] ; Open Project Program of State Key Laboratory of Theoretical Physics, Institute of Theoretical Physics, Chinese Academy of Science, China [Y5KF211CJ1] |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.itp.ac.cn/handle/311006/22094 |
| Collection | SCI期刊论文 |
| Corresponding Author | Geng, YZ (reprint author), Hebei Univ Technol, Sch Sci, Tianjin 300401, Peoples R China.; Ji, Q (reprint author), Chinese Acad Sci, Inst Theoret Phys, State Key Lab Theoret Phys, Beijing 100190, Peoples R China. |
| Recommended Citation GB/T 7714 | Jin, YM,Geng, YZ,Lu, LN,et al. Anchor Effect of Interactions Between Kinesin's Nucleotide-Binding Pocket and Microtubule[J]. CELLULAR AND MOLECULAR BIOENGINEERING,2017,10(2):162-173. |
| APA | Jin, YM.,Geng, YZ.,Lu, LN.,Ma, YL.,Lu, G.,...&Ji, Q .(2017).Anchor Effect of Interactions Between Kinesin's Nucleotide-Binding Pocket and Microtubule.CELLULAR AND MOLECULAR BIOENGINEERING,10(2),162-173. |
| MLA | Jin, YM,et al."Anchor Effect of Interactions Between Kinesin's Nucleotide-Binding Pocket and Microtubule".CELLULAR AND MOLECULAR BIOENGINEERING 10.2(2017):162-173. |
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