ITP OpenIR  > 理论物理所科研产出  > SCI论文
Song, YS; Shu, YG; Zhou, X; Ou-Yang, ZC; Li, M; Li, M (reprint author), Univ Chinese Acad Sci, Sch Phys Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China.
Proofreading of DNA polymerase: a new kinetic model with higher-order terminal effects
Source PublicationJOURNAL OF PHYSICS-CONDENSED MATTER
Language英语
KeywordDna Polymerase Proofreading Kinetics Fidelity Higher-order Terminal Effects
AbstractThe fidelity of DNA replication by DNA polymerase (DNAP) has long been an important issue in biology. While numerous experiments have revealed details of the molecular structure and working mechanism of DNAP which consists of both a polymerase site and an exonuclease (proofreading) site, there were quite a few theoretical studies on the fidelity issue. The first model which explicitly considered both sites was proposed in the 1970s and the basic idea was widely accepted by later models. However, all these models did not systematically investigate the dominant factor on DNAP fidelity, i.e. the higher-order terminal effects through which the polymerization pathway and the proofreading pathway coordinate to achieve high fidelity. In this paper, we propose a new and comprehensive kinetic model of DNAP based on some recent experimental observations, which includes previous models as special cases. We present a rigorous and unified treatment of the corresponding steady-state kinetic equations of any-order terminal effects, and derive analytical expressions for fidelity in terms of kinetic parameters under bio-relevant conditions. These expressions offer new insights on how the higher-order terminal effects contribute substantially to the fidelity in an order-by-order way, and also show that the polymerization-and-proofreading mechanism is dominated only by very few key parameters. We then apply these results to calculate the fidelity of some real DNAPs, which are in good agreements with previous intuitive estimates given by experimentalists.
2017
Volume29Issue:2Pages:25101
Subject AreaPhysics
DOIhttp://dx.doi.org/10.1088/0953-8984/29/2/025101
Funding OrganizationNational Basic Research Program of China (973 program) [2013CB932804] ; National Basic Research Program of China (973 program) [2013CB932804] ; National Basic Research Program of China (973 program) [2013CB932804] ; National Basic Research Program of China (973 program) [2013CB932804] ; National Natural Science Foundation of China [11105218, 91027046, 11574329, 11322543, 11675180] ; National Natural Science Foundation of China [11105218, 91027046, 11574329, 11322543, 11675180] ; National Natural Science Foundation of China [11105218, 91027046, 11574329, 11322543, 11675180] ; National Natural Science Foundation of China [11105218, 91027046, 11574329, 11322543, 11675180]
Citation statistics
Document Type期刊论文
Identifierhttp://ir.itp.ac.cn/handle/311006/22149
Collection理论物理所科研产出_SCI论文
Corresponding AuthorLi, M (reprint author), Univ Chinese Acad Sci, Sch Phys Sci, 19A Yuquan Rd, Beijing 100049, Peoples R China.
Recommended Citation
GB/T 7714
Song, YS,Shu, YG,Zhou, X,et al. Proofreading of DNA polymerase: a new kinetic model with higher-order terminal effects[J]. JOURNAL OF PHYSICS-CONDENSED MATTER,2017,29(2):25101.
APA Song, YS,Shu, YG,Zhou, X,Ou-Yang, ZC,Li, M,&Li, M .(2017).Proofreading of DNA polymerase: a new kinetic model with higher-order terminal effects.JOURNAL OF PHYSICS-CONDENSED MATTER,29(2),25101.
MLA Song, YS,et al."Proofreading of DNA polymerase: a new kinetic model with higher-order terminal effects".JOURNAL OF PHYSICS-CONDENSED MATTER 29.2(2017):25101.
Files in This Item:
File Name/Size DocType Version Access License
Proofreading of DNA (2168KB) 开放获取--Application Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Song, YS]'s Articles
[Shu, YG]'s Articles
[Zhou, X]'s Articles
Baidu academic
Similar articles in Baidu academic
[Song, YS]'s Articles
[Shu, YG]'s Articles
[Zhou, X]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Song, YS]'s Articles
[Shu, YG]'s Articles
[Zhou, X]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.