Knowledge Management System of Institute of Theoretical Physics, CAS
Zhang, Q; Li, R; Li, JB; Shi, HL | |
Optimal Allocation of Bacterial Protein Resources under Nonlethal Protein Maturation Stress | |
发表期刊 | BIOPHYSICAL JOURNAL |
语种 | 英语 |
关键词 | ESCHERICHIA-COLI PROTEOME SIGMA-FACTOR COMPETITION GENE-EXPRESSION RNA-POLYMERASE OXIDATIVE STRESS GROWTH-INHIBITION CELL-GROWTH TRANSLATION RATES OVERPRODUCTION |
摘要 | Under different environmental stresses, bacteria optimize the allocation of cellular resources through a variety of mechanisms. Recently, researchers have used phenomenological models to quantitatively characterize the allocation of bacterial protein resources under metabolic and translational limitations. Some stresses interfere with protein maturation, thereby enhancing the expression of chaperones and proteases. However, the reallocation of protein resources caused by such environmental stresses has not been modeled quantitatively. Here, we developed a dynamic model of coarse-grained protein resource fluxes based on a self-replicator that includes protein maturation and degradation. Through flux balance analysis, it produces a constrained optimization problem that can be solved analytically. Accordingly, we predicted protein allocation fractions as functions of growth rate under different limitations, which are basically in line with empirical data. We cultured Escherichia coli in media containing different concentrations of chloramphenicol, acetic acid, and paraquat and measured the functional relationship between the expression level of beta-galactosidase driven by a constitutive promoter and the bacterial growth rate, respectively. Taking into account the possible mode of stress limitation on the fluxes, our model reproduces this experimentally measured relationship. In addition, our model is in good agreement with the experimental relationship between growth rate and proteome fraction of unnecessary protein in E. coli, considering the unoptimized upregulation of chaperones with useless protein overexpression. The results provide a more systematic view of bacterial stress adaptation that may help in designing for bioengineering or medical interventions. |
2018 | |
ISSN | 0006-3495 |
卷号 | 115期号:5页码:896-910 |
学科领域 | Biophysics |
学科门类 | Biophysics |
DOI | 10.1016/j.bpj.2018.07.021 |
收录类别 | SCIE |
引用统计 | |
文献类型 | 期刊论文 |
条目标识符 | http://ir.itp.ac.cn/handle/311006/22826 |
专题 | SCI期刊论文 |
作者单位 | 1.Chinese Acad Sci, Inst Theoret Phys, Key Lab Theoret Phys, Beijing, Peoples R China 2.Chinese Acad Sci, Inst Chem, Beijing, Peoples R China 3.Univ Chinese Acad Sci, Sch Phys Sci, Beijing, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang, Q,Li, R,Li, JB,et al. Optimal Allocation of Bacterial Protein Resources under Nonlethal Protein Maturation Stress[J]. BIOPHYSICAL JOURNAL,2018,115(5):896-910. |
APA | Zhang, Q,Li, R,Li, JB,&Shi, HL.(2018).Optimal Allocation of Bacterial Protein Resources under Nonlethal Protein Maturation Stress.BIOPHYSICAL JOURNAL,115(5),896-910. |
MLA | Zhang, Q,et al."Optimal Allocation of Bacterial Protein Resources under Nonlethal Protein Maturation Stress".BIOPHYSICAL JOURNAL 115.5(2018):896-910. |
条目包含的文件 | ||||||
文件名称/大小 | 文献类型 | 版本类型 | 开放类型 | 使用许可 | ||
Optimal Allocation o(765KB) | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | 请求全文 |
个性服务 |
推荐该条目 |
保存到收藏夹 |
查看访问统计 |
导出为Endnote文件 |
谷歌学术 |
谷歌学术中相似的文章 |
[Zhang, Q]的文章 |
[Li, R]的文章 |
[Li, JB]的文章 |
百度学术 |
百度学术中相似的文章 |
[Zhang, Q]的文章 |
[Li, R]的文章 |
[Li, JB]的文章 |
必应学术 |
必应学术中相似的文章 |
[Zhang, Q]的文章 |
[Li, R]的文章 |
[Li, JB]的文章 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论