ITP OpenIR  > SCI期刊论文
Zhang, Q; Li, R; Li, JB; Shi, HL
Optimal Allocation of Bacterial Protein Resources under Nonlethal Protein Maturation Stress
Source PublicationBIOPHYSICAL JOURNAL
Language英语
KeywordESCHERICHIA-COLI PROTEOME SIGMA-FACTOR COMPETITION GENE-EXPRESSION RNA-POLYMERASE OXIDATIVE STRESS GROWTH-INHIBITION CELL-GROWTH TRANSLATION RATES OVERPRODUCTION
AbstractUnder different environmental stresses, bacteria optimize the allocation of cellular resources through a variety of mechanisms. Recently, researchers have used phenomenological models to quantitatively characterize the allocation of bacterial protein resources under metabolic and translational limitations. Some stresses interfere with protein maturation, thereby enhancing the expression of chaperones and proteases. However, the reallocation of protein resources caused by such environmental stresses has not been modeled quantitatively. Here, we developed a dynamic model of coarse-grained protein resource fluxes based on a self-replicator that includes protein maturation and degradation. Through flux balance analysis, it produces a constrained optimization problem that can be solved analytically. Accordingly, we predicted protein allocation fractions as functions of growth rate under different limitations, which are basically in line with empirical data. We cultured Escherichia coli in media containing different concentrations of chloramphenicol, acetic acid, and paraquat and measured the functional relationship between the expression level of beta-galactosidase driven by a constitutive promoter and the bacterial growth rate, respectively. Taking into account the possible mode of stress limitation on the fluxes, our model reproduces this experimentally measured relationship. In addition, our model is in good agreement with the experimental relationship between growth rate and proteome fraction of unnecessary protein in E. coli, considering the unoptimized upregulation of chaperones with useless protein overexpression. The results provide a more systematic view of bacterial stress adaptation that may help in designing for bioengineering or medical interventions.
2018
ISSN0006-3495
Volume115Issue:5Pages:896-910
Subject AreaBiophysics
MOST Discipline CatalogueBiophysics
DOI10.1016/j.bpj.2018.07.021
Indexed BySCIE
Citation statistics
Document Type期刊论文
Identifierhttp://ir.itp.ac.cn/handle/311006/22826
CollectionSCI期刊论文
Affiliation1.Chinese Acad Sci, Inst Theoret Phys, Key Lab Theoret Phys, Beijing, Peoples R China
2.Chinese Acad Sci, Inst Chem, Beijing, Peoples R China
3.Univ Chinese Acad Sci, Sch Phys Sci, Beijing, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Q,Li, R,Li, JB,et al. Optimal Allocation of Bacterial Protein Resources under Nonlethal Protein Maturation Stress[J]. BIOPHYSICAL JOURNAL,2018,115(5):896-910.
APA Zhang, Q,Li, R,Li, JB,&Shi, HL.(2018).Optimal Allocation of Bacterial Protein Resources under Nonlethal Protein Maturation Stress.BIOPHYSICAL JOURNAL,115(5),896-910.
MLA Zhang, Q,et al."Optimal Allocation of Bacterial Protein Resources under Nonlethal Protein Maturation Stress".BIOPHYSICAL JOURNAL 115.5(2018):896-910.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Zhang, Q]'s Articles
[Li, R]'s Articles
[Li, JB]'s Articles
Baidu academic
Similar articles in Baidu academic
[Zhang, Q]'s Articles
[Li, R]'s Articles
[Li, JB]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Zhang, Q]'s Articles
[Li, R]'s Articles
[Li, JB]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.