Quantitative Connection between Cell Size and Growth Rate by Phospholipid Metabolism

doi:10.3390/cells9020391

ITP OpenIR > SCI期刊论文

	Zhang, Zhichao 1; Zhang, Qing 1; Guan, Shaohua 1; Shi, Hualin1
	Quantitative Connection between Cell Size and Growth Rate by Phospholipid Metabolism
Source Publication	CELLS
Language	英语
Keyword	HAEMOPHILUS-INFLUENZAE RD GRAM-NEGATIVE BACTERIA ESCHERICHIA-COLI OUTER-MEMBRANE FATTY-ACID GUANOSINE TETRAPHOSPHATE CARDIOLIPIN SYNTHASE ADAPTIVE EVOLUTION ENVELOPE MODEL
Abstract	The processes involved in cell growth are extremely complicated even for a single cell organism such as Escherichia coli, while the relationship between growth rate and cell size is simple. We aimed to reveal the systematic link between them from the aspect of the genome-scale metabolic network. Since the growth rate reflects metabolic rates of bacteria and the cell size relates to phospholipid synthesis, a part of bacterial metabolic networks, we calculated the cell length from the cardiolipin synthesis rate, where the cardiolipin synthesis reaction is able to represent the phospholipid metabolism of Escherichia coli in the exponential growth phase. Combined with the flux balance analysis, it enables us to predict cell length and to examine the quantitative relationship between cell length and growth rate. By simulating bacteria growing in various nutrient media with the flux balance analysis and calculating the corresponding cell length, we found that the increase of the synthesis rate of phospholipid, the cell width, and the protein fraction in membranes caused the increase of cell length with growth rate. Different tendencies of phospholipid synthesis rate changing with growth rate result in different relationships between cell length and growth rate. The effects of gene deletions on cell size and growth rate are also examined. Knocking out the genes, such as Delta tktA, Delta tktB, Delta yqaB, Delta pgm, and Delta cysQ, affects growth rate largely while affecting cell length slightly. Results of this method are in good agreement with experiments.
	2020
Volume	9 Issue:2 Pages:391
Cooperation Status	国内
Subject Area	Cell Biology
MOST Discipline Catalogue	Cell Biology
DOI	10.3390/cells9020391
Indexed By	SCIE
Citation statistics	Cited Times:1[WOS] [WOS Record] [Related Records in WOS]
Document Type	期刊论文
Identifier	http://ir.itp.ac.cn/handle/311006/27074
Collection	SCI期刊论文
Affiliation	1.Univ Chinese Acad Sci, Sch Phys Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Inst Theoret Phys, CAS Key Lab Theoret Phys, Beijing 100190, Peoples R China
Recommended Citation GB/T 7714	Zhang, Zhichao,Zhang, Qing,Guan, Shaohua,et al. Quantitative Connection between Cell Size and Growth Rate by Phospholipid Metabolism[J]. CELLS,2020,9(2):391.
APA	Zhang, Zhichao,Zhang, Qing,Guan, Shaohua,&Shi, Hualin.(2020).Quantitative Connection between Cell Size and Growth Rate by Phospholipid Metabolism.CELLS,9(2),391.
MLA	Zhang, Zhichao,et al."Quantitative Connection between Cell Size and Growth Rate by Phospholipid Metabolism".CELLS 9.2(2020):391.

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