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题名: The effect of mechanical tension on DNA polymerase activity studied with a two-state model
作者: Shu, Yaogen ;  Shi, Hualin
刊名: MODERN PHYSICS LETTERS B
出版日期: 2007
卷号: 21, 期号:17, 页码:1097-1105
关键词: SINGLE-STRANDED-DNA ;  REPLICATION FIDELITY ;  EXONUCLEASE ;  COMPLEX
学科分类: Physics
通讯作者: Shu, YG , Chinese Acad Sci, Inst Theoret Phys, POB 2735, Beijing 100080, Peoples R China
部门归属: [Shu, YG; Shi, HL] Chinese Acad Sci, Inst Theoret Phys, Beijing 100080, Peoples R China
英文摘要: Single-molecule experiments revealed that the replication rate of T7 DNA polymerase has a peak value at tension of about 5.5 pN. However, the mechanism leading to this peak has only been partially investigated. Here, we present a two-state model to investigate the effect of mechanical tension on DNA polymerase activity. The model consists of polymerase and exonuclease. The polymerase's kinetic pathway has been simplified into Michaelis-Menten form with two steps. One is the conformational change from "open" to "close". The other is the base-stacking. The results are in good agreement with experimental observations. We also predict that the tension on template is beneficial to the fidelity of DNA replication.
收录类别: SCI
原文出处: 查看原文
WOS记录号: WOS:000251911900006
Citation statistics: 
内容类型: 期刊论文
URI标识: http://ir.itp.ac.cn/handle/311006/5693
Appears in Collections:理论物理所1978-2010年知识产出_期刊论文

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Recommended Citation:
Shu, Yaogen,Shi, Hualin. The effect of mechanical tension on DNA polymerase activity studied with a two-state model[J]. MODERN PHYSICS LETTERS B,2007,21(17):1097-1105.
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