ITP OpenIR  > 理论物理所1978-2010年知识产出
The effect of mechanical tension on DNA polymerase activity studied with a two-state model
Shu, Yaogen; Shi, Hualin; Shu, YG , Chinese Acad Sci, Inst Theoret Phys, POB 2735, Beijing 100080, Peoples R China
2007
发表期刊MODERN PHYSICS LETTERS B
ISSN0217-9849
卷号21期号:17页码:1097-1105
摘要Single-molecule experiments revealed that the replication rate of T7 DNA polymerase has a peak value at tension of about 5.5 pN. However, the mechanism leading to this peak has only been partially investigated. Here, we present a two-state model to investigate the effect of mechanical tension on DNA polymerase activity. The model consists of polymerase and exonuclease. The polymerase's kinetic pathway has been simplified into Michaelis-Menten form with two steps. One is the conformational change from "open" to "close". The other is the base-stacking. The results are in good agreement with experimental observations. We also predict that the tension on template is beneficial to the fidelity of DNA replication.
部门归属[Shu, YG; Shi, HL] Chinese Acad Sci, Inst Theoret Phys, Beijing 100080, Peoples R China
关键词Single-stranded-dna Replication Fidelity Exonuclease Complex
学科领域Physics
URL查看原文
收录类别SCI
WOS记录号WOS:000251911900006
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.itp.ac.cn/handle/311006/5693
专题理论物理所1978-2010年知识产出
通讯作者Shu, YG , Chinese Acad Sci, Inst Theoret Phys, POB 2735, Beijing 100080, Peoples R China
推荐引用方式
GB/T 7714
Shu, Yaogen,Shi, Hualin,Shu, YG , Chinese Acad Sci, Inst Theoret Phys, POB 2735, Beijing 100080, Peoples R China. The effect of mechanical tension on DNA polymerase activity studied with a two-state model[J]. MODERN PHYSICS LETTERS B,2007,21(17):1097-1105.
APA Shu, Yaogen,Shi, Hualin,&Shu, YG , Chinese Acad Sci, Inst Theoret Phys, POB 2735, Beijing 100080, Peoples R China.(2007).The effect of mechanical tension on DNA polymerase activity studied with a two-state model.MODERN PHYSICS LETTERS B,21(17),1097-1105.
MLA Shu, Yaogen,et al."The effect of mechanical tension on DNA polymerase activity studied with a two-state model".MODERN PHYSICS LETTERS B 21.17(2007):1097-1105.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
The effect of mechan(199KB) 开放获取使用许可请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Shu, Yaogen]的文章
[Shi, Hualin]的文章
[Shu, YG , Chinese Acad Sci, Inst Theoret Phys, POB 2735, Beijing 100080, Peoples R China]的文章
百度学术
百度学术中相似的文章
[Shu, Yaogen]的文章
[Shi, Hualin]的文章
[Shu, YG , Chinese Acad Sci, Inst Theoret Phys, POB 2735, Beijing 100080, Peoples R China]的文章
必应学术
必应学术中相似的文章
[Shu, Yaogen]的文章
[Shi, Hualin]的文章
[Shu, YG , Chinese Acad Sci, Inst Theoret Phys, POB 2735, Beijing 100080, Peoples R China]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。