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Shu, Yaogen; Shi, Hualin; Shu, YG , Chinese Acad Sci, Inst Theoret Phys, POB 2735, Beijing 100080, Peoples R China
The effect of mechanical tension on DNA polymerase activity studied with a two-state model
Source PublicationMODERN PHYSICS LETTERS B
KeywordSingle-stranded-dna Replication Fidelity Exonuclease Complex
AbstractSingle-molecule experiments revealed that the replication rate of T7 DNA polymerase has a peak value at tension of about 5.5 pN. However, the mechanism leading to this peak has only been partially investigated. Here, we present a two-state model to investigate the effect of mechanical tension on DNA polymerase activity. The model consists of polymerase and exonuclease. The polymerase's kinetic pathway has been simplified into Michaelis-Menten form with two steps. One is the conformational change from "open" to "close". The other is the base-stacking. The results are in good agreement with experimental observations. We also predict that the tension on template is beneficial to the fidelity of DNA replication.
2007
ISSN0217-9849
Volume21Issue:17Pages:1097-1105
Subject AreaPhysics
Indexed BySCI
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Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.itp.ac.cn/handle/311006/5693
Collection理论物理所科研产出_SCI论文
Corresponding AuthorShu, YG , Chinese Acad Sci, Inst Theoret Phys, POB 2735, Beijing 100080, Peoples R China
Recommended Citation
GB/T 7714
Shu, Yaogen,Shi, Hualin,Shu, YG , Chinese Acad Sci, Inst Theoret Phys, POB 2735, Beijing 100080, Peoples R China. The effect of mechanical tension on DNA polymerase activity studied with a two-state model[J]. MODERN PHYSICS LETTERS B,2007,21(17):1097-1105.
APA Shu, Yaogen,Shi, Hualin,&Shu, YG , Chinese Acad Sci, Inst Theoret Phys, POB 2735, Beijing 100080, Peoples R China.(2007).The effect of mechanical tension on DNA polymerase activity studied with a two-state model.MODERN PHYSICS LETTERS B,21(17),1097-1105.
MLA Shu, Yaogen,et al."The effect of mechanical tension on DNA polymerase activity studied with a two-state model".MODERN PHYSICS LETTERS B 21.17(2007):1097-1105.
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